Section 1

Diagnostic Procedures for Finfish and Shellfish Pathogens

Chapter 1 Bacterial Diseases of Fish

1.1 GENERAL PROCEDURES

1.1.1 General Procedures for Bacteriology
1.1.2 Flow Chart for the Presumptive Identification of Selected Bacteria from Fishes

1.2 GRAM-NEGATIVE BACTERIAL DISEASES

1.2.1 Bacterial Gill Disease
1.2.2 Coldwater Disease
1.2.3 Columnaris Disease (Video 1 )  (Video 2)
1.2.4 Edwardsiella tarda Septicemia
1.2.5 Enteric Redmouth Disease
1.2.6 Enteric Septicemia
1.2.7 Furunculosis
1.2.8 Other Diseases Caused by Aeromonas salmonicida
1.2.9 Motile Aeromonas Septicemia 
1.2.10 Vibriosis
1.2.11 Coldwater Vibriosis
1.2.12 Francisellosis 
           1.2.12.1 Worksheet A
           1.2.12.2 Worksheet B
1.2.13 Piscirickettsiosis
1.2.14 Photobacteriosis

1.3 GRAM-POSITIVE BACTERIAL DISEASES

1.3.1 Bacterial Kidney Disease
         1.3.1.1 BKD Methods Appendix 1
         1.3.1.2 BKD Methods Appendix 2
1.3.2 Pseudokidney Disease
1.3.3 Streptococcal Disease
1.3.4 Weissellosis

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Chapter 2 Viral Diseases of Fish

2.1 GENERAL PROCEDURES FOR VIROLOGY

2.1.1 General Procedures for Virology
2.1.2 General Procedures for Electron Microscopy Applications in Diagnostic Virology

2.2 SPECIFIC VIRAL DISEASES

2.2.1 Channel Catfish Virus Disease
2.2.2 Erythrocytic Inclusion Body Syndrome
2.2.3 Herpesvirus Diseases of Salmonids
2.2.4 Infectious Hematopoietic Necrosis
2.2.5 Infectious Pancreatic Necrosis
2.2.6 Viral Erythrocytic Necrosis 
2.2.7 Viral Hemorrhagic Septicemia
2.2.8 Infectious Salmon Anemia
         2.2.8.1 ISA Appendix 1
         2.2.8.2 ISA Appendix 2
2.2.9 Spring Viremia of Carp
2.2.10 Koi Herpesvirus Disease
2.2.11 Goldfish Herpesviral Hematopoietic Necrosis
2.2.12 Largemouth Bass Virus Disease
2.2.13 Oncorhynchus masou virus Disease

2.3 OTHER VIRAL DISEASES

2.3.1 Other Viruses Isolated from Fish

2.A1 APPENDIX 1: METHODS FOR TESTING FOR THE PRESENCE OF MYCOPLASMA IN STOCK CELL CULTURES

2.A.1 Appendix 1: Methods for Mycoplasma

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Chapter 3 Parasitic Diseases of Fish

3.1 GENERAL PROCEDURES

3.1.1 General Procedures for Parasitology
3.1.2 Key to Major Taxa of Adult Parasites of Fishes

3.2 SPECIFIC PARASITIC DISEASES

3.2.1 Ichthyobodiasis
3.2.2 Diplomonad (Hexamitid) Flagellates: Diplomonadiasis, Hexamitiasis, Spironucleosis
3.2.3 Ovipleistophoriasis: A Microsporidian Disease of the Golden Shiner Ovary
3.2.4 Salmonid Ceratomyxosis
         3.2.4.1 Salmonid Ceratomyxosis Methods Appendix 1
3.2.5 Whirling Disease of Salmonids
3.2.6 Proliferative Gill Disease
        3.2.6.1 Proliferative Gill Disease Methods Appendix 1
3.2.7 Proliferative Kidney Disease
3.2.8 Ichthyophthiriasis
3.2.9 External Infection by Ciliated Parasites
3.2.10 Monogenean Diseases
3.2.11 Bothriocephalosis   (video)
3.2.12 Lernaeid Parasitism
3.2.13 Gill Maggot Disease (Genus Salmincola)
3.2.14 Sea Lice Parasitism
3.2.15 Branchiuran Fish Louse Disease
3.2.16 Centrocestiasis  (Video 3)  (Video 4)  (Video 5b)  (Video 6b)  (Video 11)  (Video 13)
3.2.17 Salmonid Intranuclear Microsporosis (Nucleospora salmonis)
           3.2.17.1 Nested PCR: Nucleospora (Enterocytozoon) salmonis
           3.2.17.2 Quantitative PCR: Nucleospora (Enterocytozoon) salmonis
3.2.18 Ichthyophonus Disease

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Chapter 4 Mycotic Diseases of Fish

4.1 GENERAL PROCEDURES FOR MYCOLOGY

4.1 General Procedures for Mycology

4.2 SPECIFIC DISEASES

4.2.1 Branchiomycosis
4.2.2 Saprolegniasis

4.3 MISCELLANEOUS MYCOTIC DISEASES

4.3 Miscellaneous Mycotic Diseases

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Chapter 5 Diseases of Molluscan Shellfish

5.1 DISEASES OF SHELLFISH INTRODUCTION

5.1 Diseases of Shellfish Introduction

5.2 SPECIFIC DISEASES

5.2.1 Perkinsus sp. Infections of Marine Molluscs
5.2.2 Haplosporidiosis of American Oysters
5.2.3 Oyster Seaside Haplosporidiosis
5.2.4 Velar Virus Disease of Pacific Oysters
5.2.5 Mikrocytosis (Denman Island Disease)
5.2.6 Nocardiosis of Pacific Oysters
5.2.7 Bonamiasis of Ostreid Oysters
5.2.8 Hexamitiasis of Oysters
5.2.9 Marteiliasis of Ostrea edulis
5.2.10 Shell Disease of Oysters
5.2.11 Disseminated Neoplasia of Bivalve Molluscs
5.2.12 Miscellaneous Diseases of Molluscs
5.2.13 Juvenille Oyster Disease

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Chapter 6 Diseases of Crustaceans

6.2 SPECIFIC DISEASES

6.2.1 Necrotizing Hepatopancreatitis Disease of Peneaid Shrimp
6.2.2 Taura Syndrome of Peneaid Shrimp
6.2.3 Lagenidiasis of Decapod Crustaceans
6.2.4 White Spot Syndrome
6.2.5 Infection with Hematodinium